Rabbit anti-Mouse IgG (H&L), ALP conjugated

Product Specifications

Background

Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Mouse IgG heavy and light chains (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified Mouse IgG, whole molecule

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)

Field of Research

Anti-Mouse

Dilution

1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Purity

Immunogen affinity purified rabbit IgG.

Format

Liquid, clear, colorless.

Precautions

Based on IEP, this antibody binds to:heavy chains on mouse IgGlight chains on all mouse immunoglobulinsNo reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

Additionnal Information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)

Storage Conditions

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.