Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample
Product Specifications
Background
Rabbit anti-mouse IgGis a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins inimmunological assays.
Host
Rabbit
Reactivity
Mouse IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Mouse IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
Purity
Immunogen affinity purified rabbit IgG.
Format
Liquid
Precautions
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
Storage Conditions
Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Product Datasheet
https://cdn.gentaur.com/products/451/686bf4632da8eae897d64f01/datasheet/as09 627-trial.pdf
CAS Number
9007-83-4
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