Rabbit anti-Mouse IgG (H&L), HRP conjugated - trial sample
Selected Variant
AS09 627-trial
Size: 10 µL (0.91 mg/mL)
Product Specifications
Background
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
Mouse IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Mouse IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Mouse
Dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 5 000 (WB)
Purity
Immunogen affinity purified rabbit IgG.
Format
Liquid
Precautions
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis
Additionnal Information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Storage Conditions
Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Prediction Reactivity
Mouse IgG Heavy and Light chains (H&L)
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