Goat anti-Rat IgG (H&L), ALP conjugated
Product Specifications
Background
Goat anti-rat IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Rat IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified Rat IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Rat
Dilution
1 : 500-1 :2000 (ELISA), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Purity
Immunogen affinity purified goat IgG.
Format
Liquid
Precautions
No reactivity is observed to non-immunoglobulin rat serum proteins based in immunoelectrophoresis, No reacticity to mouse and human serum proteins based on immunoelectrophoresis
Additionnal Information
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Storage Conditions
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Prediction Reactivity
Rat IgG Heavy and Light chains (H&L)
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