Donkey anti-Sheep IgG (H&L), Biotin conjugated
Product Specifications
Background
Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Donkey
Reactivity
Sheep IgG heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified sheep IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Sheep
Dilution
1 : 2000-1: 20 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 2000-1: 20 000 (WB)
Purity
Immunogen purified donkey IgG.
Format
Liquid
Precautions
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Additionnal Information
Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L) biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Storage Conditions
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Prediction Reactivity
Sheep IgG Heavy and Light chains (H&L)
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