Goat anti-Rabbit IgG (H&L), ALP conjugated

Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.

Based upon IEP, this antibody binds to:• Heavy chains on rabbit IgG• Light chains on all rabbit immunoglobulinsNo reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Concentration: 1.5mg/ml.Antibody has been affinity purified on solid phase rabbit IgG (H&L) .AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

Pinczés et al. (2024) . Viral coat proteins decrease the gene silencing activity of cognate and heterologous viral suppressors. Sci Rep. 2024 Dec 28;14 (1) :31008. doi: 10.1038/s41598-024-81998-4. Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22 (1) :151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Bapatla et al. (2021) . Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum) . Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Szymanska et al. (2019) . SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20 (1) . pii: E143. doi: 10.3390/ijms20010143.Rozpadek et al. (2018) . Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.Borovik and Grabelnych (2018) . Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.