Goat anti-Chicken IgY (H&L), ALP conjugated
Product Specifications
Background
Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Chicken IgY heavy and light chains (H&L)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Purified chicken IgY, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Chicken
Dilution
1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB)
Purity
Immunogen affinity purified goat IgG.
Format
Liquid
Precautions
No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresischicken IgY is also called chicken or hen IgG
Additionnal Information
Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0) Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L) Antibody is affinity purified using solid phase Chicken IgG/Y.AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
Storage Conditions
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Prediction Reactivity
Chicken IgY Heavy and Light chains (H&L)
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