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Rabbit anti-Goat IgG (H&L), HRP conjugated - trial sample

Product Specifications

Background

Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Goat IgG heavy and light chains (H&L)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Purified goat IgG, whole molecule

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

Anti-Goat

Dilution

1 : 10 000 -1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000 - 50 000 (WB)

Purity

Immunogen affinity purified rabbit IgG.

Format

Liquid

Precautions

No reactivity is observed to non-immunoglobulin goat  serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Additionnal Information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free0,1 % (v/v) of Kathon CG is used as preservative

Storage Conditions

Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Prediction Reactivity

Goat IgG Heavy and Light chains (H&L)

Frequently Asked Questions

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