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Goat anti-Rabbit IgG (H&L), HRP conjugated

Product Specifications

Background

Goat anti-rabbit IgG(H&L)is a secondary antibody conjugated to HRP which binds to all rabbit IgGs inimmunological assays.

Host

Goat

Reactivity

Based on IEP, this antibody reacts with: rabbit IgG heavy chains and light chains on all rabbit immunoglobulins

Not reactive in

Non-immunoglobulin rabbit serum proteins

Immunogen

Purified Rabbit IgG, whole molecule,

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Dilution

1 : 50 000 -1 : 90 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1: 10 000 -1 : 50 000 (WB)

Purity

Immunogen affinity purified using solid phase rabbit IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily

References & Citations

Caballeroet al. (2024). Connecting high-resolution 3D chromatin maps with cell division and cell differentiation at the root apical meristem. Plant Cell Rep. 2024 Sep 16;43(10):232. doi: 10.1007/s00299-024-03322-8.Truonget al. (2024). Apo-siderophores promote growth of iron-deficient Arabidopsis plants by mobilizing iron from roots to shoots and reducing oxidative stress in roots. Plant Stress, Volume 12, June 2024, 100488.Martín-Merchánet al. (2024). Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387.Miloroet al. (2024). Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation. Plant Cell Rep. 2024 Mar 13;43(4):96. doi: 10.1007/s00299-024-03177-z.Liuet al. (2023). RBPome identification in egg-cell like callus of Arabidopsis. Biol Chem. 2023 Sep 29;404(11-12):1137-1149.doi: 10.1515/hsz-2023-0195.Chunget al. (2023). An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression. Nucleic Acids Res. 2023 Nov 10;51(20):11386-11400. doi: 10.1093/nar/gkad816.Shiet al. (2023). Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity labeling. STAR Protoc. 2023 Sep 20;4(4):102577.doi: 10.1016/j.xpro.2023.102577.Limet al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Miklankovaet al. (2022) HYPK promotes the activity of the Nalfa-acetyltransferase A complex to determine proteostasis of nonAc-X2/N-degron-containing proteins. Sci Adv. 2022 Jun 17;8(24):eabn6153. doi: 10.1126/sciadv.abn6153. Epub 2022 Jun 15. PMID: 35704578; PMCID: PMC9200280.Hofmann,Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Bychkovet al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Vitaleet al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.Larssonet al. (2023) Dynamin2 functions as an accessory protein to reduce the rate of caveola internalizationZhanget al. (2023). Structural insights into a unique PSI-LHCI-LHCII-Lhcb9 supercomplex from moss Physcomitrium patensTükenmezet al (2023). A Highly Substituted Ring-Fused 2-Pyridone Compound Targeting PrfA and the Efflux Regulator BrtA in Listeria monocytogenes. mBio. 2023 Jun 27;14(3):e0044923. doi: 10.1128/mbio.00449-23Aguiló-Nicolauet al. (2023) Singular adaptations in the carbon assimilation mechanism of the polyextremophile cyanobacterium Chroococcidiopsis thermalis. Photosynth Res. 2023 May;156(2):231-245. doi: 10.1007/s11120-023-01008-y.Žemaitiset al. (2023 The stem cell-supporting small molecule UM171 triggers Cul3-KBTBD4-mediated degradation of ELM2 domain-harboring proteins. J Biol Chem. 2023 May;299(5):104662. doi: 10.1016/j.jbc.2023.104662.Cembrowska-Lech, Rybak (2023) Nanopriming of Barley Seeds-A Shotgun Approach to Improve Germination under Salt Stress Conditions by Regulating of Reactive Oxygen Species. Plants (Basel). 2023;12(2):405. Published 2023 Jan 15. doi:10.3390/plants12020405Miernickaet al. (2022) The Adjustment Strategy of Venus Flytrap Photosynthetic Apparatus to UV-A Radiation. Cells. 2022;11(19):3030. Published 2022 Sep 27. doi:10.3390/cells11193033Boussardon, Bag, Juvany, et al. (2022) The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence. Commun Biol. 2022;5(1):1043. Published 2022 Sep 30. doi:10.1038/s42003-022-03998-4Linsteret al. (2022). Cotranslational N-degron masking by acetylation promotes proteome stability in plants. Nat Commun. 2022 Feb 10;13(1):810. doi: 10.1038/s41467-022-28414-5. PMID: 35145090; PMCID: PMC8831508.Baenaet al. (2022) SNARE SYP132 mediates divergent traffic of plasma membrane H+-ATPase AHA1 and antimicrobial PR1 during bacterial pathogenesis. Plant Physiol. 2022 Jun 27;189(3):1639-1661. doi: 10.1093/plphys/kiac149. PMID: 35348763; PMCID: PMC9237740.Franziskaet al. (2022) Auxin application to maize plants at flowering increases abundance and activity of plasma membrane H+-ATPase in developing maize kernels, Journal of Plant Nutrition and Soil Science, Volume 185, Issue 5, October 2022.Ye, Zhou, Zhu, et al. (2022) Inhibition of shoot-expressed NRT1.1 improves reutilization of apoplastic iron under iron-deficient conditions. Plant J. 2022;112(2):549-564. doi:10.1111/tpj.15967Perera-Castroet al (2022). Limitations to photosynthesis in bryophytes: certainties and uncertainties regarding methodology. J Exp Bot. 2022;73(13):4592-4604. doi:10.1093/jxb/erac191Limet al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Miklankovaet al. (2022) HYPK promotes the activity of the Nalfa-acetyltransferase A complex to determine proteostasis of nonAc-X2/N-degron-containing proteins. Sci Adv. 2022 Jun 17;8(24):eabn6153. doi: 10.1126/sciadv.abn6153. Epub 2022 Jun 15. PMID: 35704578; PMCID: PMC9200280.Hofmann,Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Bychkovet al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Vitaleet al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.Gucumet al. (2021) A patient-based medaka alg2 mutant as a model for hypo-N-glycosylation. Development. 2021 Jun 1;148(11):dev199385. doi: 10.1242/dev.199385. Epub 2021 Jun 9. PMID: 34106226; PMCID: PMC8217707.Wuet al. (2021). Formation of light-harvesting complex (LHC) II aggregates from LHCII-PSI-LHCI complexes in rice plants under high light. J Exp Bot. 2021 May 3:erab188. doi: 10.1093/jxb/erab188. Epub ahead of print. PMID: 33939808.Wojciechowskaet al. (2021) Localization and Dynamics of the Methionine Sulfoxide Reductases MsrB1 and MsrB2 in Beech Seeds. Int J Mol Sci. 2021 Jan 2;22(1):402. doi: 10.3390/ijms22010402. PMID: 33401671; PMCID: PMC7795007.Longet al. (2020). FOXO3 is targeted by miR-223-3p and promotes osteogenic differentiation of bone marrow mesenchymal stem cells by enhancing autophagy. Hum Cell. 2020 Sep 13.doi: 10.1007/s13577-020-00421-y.Khajuriaet al. (2020). Photochemical Efficiency Is Negatively Correlated With the Delta 9- Tetrahydrocannabinol Content in Cannabis Sativa L. Plant Physiol Biochem. 2020 Apr 8;151:589-600. doi: 10.1016/j.plaphy.2020.04.003.Ignatovet al. (2020). An mRNA-mRNA Interaction Couples Expression of a Virulence Factor and Its Chaperone in Listeria Monocytogenes. Cell Rep, 30 (12), 4027-4040.e7Huet al. (2019). Photoprotection capacity of microalgae improved by regulating the antenna size of light-harvesting complexes. J Appl Phycol (2019). doi.org/10.1007/s10811-019-01969-5Schoberet al. (2019). Organelle Studies and Proteome Analyses on Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana. Plant Cell Physiol. 2019 Jun 10. pii: pcz097. doi: 10.1093/pcp/pcz097.Rethoreet al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325.Contreraset al. (2019). UV-B shock induces photoprotective flavonoids but not antioxidant activity in Antarctic Colobanthus quitensis (Kunth) Bartl. Environmental and Experimental Botany, Volume 159, March 2019, Pages 179-190.Nikkanenet al. (2018). Multilevel regulation of non-photochemical quenching and state transitions by chloroplast NADPH-dependent thioredoxin reductase. Physiol Plant. 2018 Dec 22. doi: 10.1111/ppl.12914.Migockaet al. (2018). Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess. Plant J. 2018 Jun 22. doi: 10.1111/tpj.14006.Tonget al. (2018). Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology. 2018 Apr 13;16(1):38. doi: 10.1186/s12951-018-0365-y.Nikkanenet al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv. doi.org/10.1101/261560.Sinclairet al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.Gzylet al. (2017). Gamma-tubulin distribution and ultrastructural changes in root cells of soybean (Glycine max L.) seedlings under cadmium stress. Environmental and Experimental Botany, Vol 143, Nov 2017, Pages 82-90.Kamieset al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.Niederhuberet al. (2017). Super-resolution microscopy of the ß-carboxysome reveals a homogenous matrix. Mol Biol Cell. 2017 Aug 9. pii: mbc.E17-01-0069. doi: 10.1091/mbc.E17-01-0069.Tyuerevaet al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Romanowskaet al. (2017). Differences in photosynthetic responses of NADP-ME type C4 species to high light. Planta. 2017 Mar;245(3):641-657. doi: 10.1007/s00425-016-2632-1. Epub 2016 Dec 18.Goodet al. (2016). Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA. Cell Chem Biol. 2016 Mar 17;23(3):404-14. doi: 10.1016/j.chembiol.2016.02.013.Dattaet al. (2016). Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol. 2015 Dec;169(4):2963-81. doi: 10.1104/pp.15.01543. Epub 2015 Oct 13.Barahimipouret al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.CiteABhas listed this antibody as one of the 7000 most published antibodies in the world.

Storage Conditions

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water, add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Product Datasheet

https://cdn.gentaur.com/products/451/686bf4632da8eae897d650d5/datasheet/as09 602.pdf

CAS Number

9007-83-4

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