Goat anti-Rabbit IgG (H&L), HRP conjugated
Product Specifications
Background
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Based on IEP, this antibody reacts with: rabbit IgG heavy chains and light chains on all rabbit immunoglobulins
Not reactive in
Non-immunoglobulin rabbit serum proteins
Immunogen
Purified Rabbit IgG, whole molecule
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Anti-Rabbit
Dilution
1 : 50 000 -1 : 90 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1: 10 000 -1 : 50 000 (WB)
Purity
Immunogen affinity purified using solid phase rabbit IgG.
Format
Lyophilized
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily
Additionnal Information
Concentration: 1.0 mg/ml.Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1% (v/v) ProClin 150.Affinity purified antibody is >95% pure, according to SDS-PAGE.This antibody can used on a very wide range of samples from various species including many model plants, algae, diatoms and bacteria.
References & Citations
Wang et al. (2025) . A receptor antagonist counterbalances multiple systemin phytocytokines in tomato. Cell . 2025 Aug 12:S0092-8674 (25) 00866-9. doi: 10.1016/j.cell.2025.07.044.Ma et al. (2025) . RACK1A positively regulates opening of the apical hook in Arabidopsis thaliana via suppression of its auxin response gradient. Proc Natl Acad Sci U S A . 2025 Jul 29;122 (30) :e2407224122. doi: 10.1073/pnas.2407224122. Dalmadi et al. (2025) . Remote precursor elements can modulate RNA induced silencing complex-loading efficiency of miR168 in Arabidopsis. Plant J . 2025 May;122 (3) :e70195. doi: 10.1111/tpj.70195. Kuang et al. (2025) . The burning glass effect of water droplets triggers a high light-induced calcium response in the chloroplast stroma. Curr Biol. 2025 Jun 9;35 (11) :2642-2658.e7. doi: 10.1016/j.cub.2025.04.065.Wójtowicz et al. (2025) . Shrink or expand? Just relax! Bidirectional grana structural dynamics as early light-induced regulator of photosynthesis. New Phytol . 2025 Jun;246 (6) :2580-2596. doi: 10.1111/nph.70175. McKenzie and Puthiyaveetil (2025) . Protein phosphorylation and oxidative protein modification promote plant photosystem II disassembly for repair. Plant Commun . 2025 Mar 10;6 (3) :101202. doi: 10.1016/j.xplc.2024.101202.Yabrag et al. (2025) .A new understanding of Acanthamoeba castellanii: dispelling the role of bacterial pore-forming toxins in cyst formation and amoebicidal actions. Cell Death Discov. 2025 Feb 19;11 (1) :66. doi: 10.1038/s41420-025-02345-8. Boussardon et al. (2025) . The atypical proteome of mitochondria from mature pollen grains. Curr Biol . 2025 Jan 21:S0960-9822 (24) 01705-6. doi: 10.1016/j.cub.2024.12.037. Pinczés et al. (2024) . Viral coat proteins decrease the gene silencing activity of cognate and heterologous viral suppressors. Sci Rep. 2024 Dec 28;14 (1) :31008. doi: 10.1038/s41598-024-81998-4. Caballero et al. (2024) . Connecting high-resolution 3D chromatin maps with cell division and cell differentiation at the root apical meristem. Plant Cell Rep. 2024 Sep 16;43 (10) :232. doi: 10.1007/s00299-024-03322-8. Truong et al. (2024) . Apo-siderophores promote growth of iron-deficient Arabidopsis plants by mobilizing iron from roots to shoots and reducing oxidative stress in roots. Plant Stress, Volume 12, June 2024, 100488. Martín-Merchán et al. (2024) . Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387. Miloro et al. (2024) . Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation. Plant Cell Rep. 2024 Mar 13;43 (4) :96. doi: 10.1007/s00299-024-03177-z. Liu et al. (2023) . RBPome identification in egg-cell like callus of Arabidopsis. Biol Chem. 2023 Sep 29;404 (11-12) :1137-1149.doi: 10.1515/hsz-2023-0195. Chung et al. (2023) . An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression. Nucleic Acids Res. 2023 Nov 10;51 (20) :11386-11400. doi: 10.1093/nar/gkad816. Shi et al. (2023) . Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity labeling. STAR Protoc. 2023 Sep 20;4 (4) :102577.doi: 10.1016/j.xpro.2023.102577. Lim et al (2022) . Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13 (1) :652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Miklankova et al. (2022) HYPK promotes the activity of the Nalfa-acetyltransferase A complex to determine proteostasis of nonAc-X2/N-degron-containing proteins. Sci Adv. 2022 Jun 17;8 (24) :eabn6153. doi: 10.1126/sciadv.abn6153. Epub 2022 Jun 15. PMID: 35704578; PMCID: PMC9200280.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel) . 2022 Apr 25;11 (5) :836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.
Storage Conditions
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water, add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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