Anti-Urease

Product Specifications

Background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Canavalia ensiformis

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Urease isolated and purified from Canavalia ensiformis (common jack-beans)

Clonality

Polyclonal

Applications

ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Field of Research

Nitrogen Metabolism

Dilution

1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)

Purity

Purified IgG in PBS.

Format

Lyophilized

Reconstitution

For reconstitution add 1 ml of sterile destilled water

Molecular Weight

91 kDa

Precautions

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Additionnal Information

Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.