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Anti-SE | Serrate RNA effector molecule (rabbit antibody)

Product Specifications

Background

Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA) .

CAS Number

9007-83-4

HS Code

38221900

Gene ID

817252

UniProt

Q9ZVD0

Host

Rabbit

Reactivity

Arabidopsis thaliana, Malus domestica, Nicotiana benthamina, Nicotiana tabacum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence Q9ZVD0, At2g27100

Clonality

Polyclonal

Applications

Chromatin Immunoprecipitation (ChIP), Immunolocalization (IL), RNA Immunoprecipitation (RIP), Western blot (WB)

Field of Research

Plant RNA

Dilution

1 : 500 (IL), 1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

81 | 80 kDa

Precautions

Suggested blotting conditions: 8% gel, tank blotting, 200 mA/ 1h to nitrocellulose membrane

References & Citations

Li et al. (2023) . JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis. Nucleic Acids Res . 2023 Nov 22:gkad1105. doi: 10.1093/nar/gkad1105. Li et al. (2021) . In vitro Reconstitution Assays of Arabidopsis 20S Proteasome. Bio-protocol 11 (7) : e3967. DOI: 10.21769/BioProtoc.3967.Li et al. (2020) . Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis. Hortic Res. 2020 Jul 1;7:98.doi: 10.1038/s41438-020-0320-6. (ChIP) Li et al. (2019) . Global co-transcriptional splicing in Arabidopsis and the correlation with splicing regulation in mature RNAs. Mol Plant. 2019 Nov 20. pii: S1674-2052 (19) 30367-3. doi: 10.1016/j.molp.2019.11.003.Wang et al. (2019) . The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis. Plant Cell. 2019 Feb;31 (2) :486-501. doi: 10.1105/tpc.18.00556. de Francisco Amorim et al. (2018) . The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing. Plant Cell. 2018 Nov;30 (11) :2838-2854. doi: 10.1105/tpc.18.00244. Epub 2018 Oct 11.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Saccharum hybrid cultivar NCo 376, Zea maysSpecies of your interest not listed? Contact us

Frequently Asked Questions

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