Anti-AGO1 | Argonaute 1 (40 µg, HRP-conjugated)
Selected Variant
AS09 527-HRP
Size: 40 µg
Product Specifications
Background
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) .This antibody is directly conjugated to Horseradish Peroxidase (HRP) .
CAS Number
9007-83-4
HS Code
38221900
Gene ID
841262
UniProt
O04379
Host
Rabbit
Reactivity
Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
Not reactive in
Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays
Immunogen
KLH-conjugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Plant RNA
Dilution
1 : 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Format
Liquid
Molecular Weight
116.4 | 130 kDa
Precautions
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Additionnal Information
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method
Storage Conditions
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Prediction Reactivity
Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
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