What is the catalog number for Anti-AKT1 | Potassium channel AKT1?

The catalog number for Anti-AKT1 | Potassium channel AKT1 is AS09 516.

What sizes are available for Anti-AKT1 | Potassium channel AKT1?

Anti-AKT1 | Potassium channel AKT1 is available in the following size(s): 200 µg.

Is Anti-AKT1 | Potassium channel AKT1 in stock?

Anti-AKT1 | Potassium channel AKT1 is currently made to order / not held in immediate stock. Contact us for lead time.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Anti-AKT1 | Potassium channel AKT1

Product Specifications

Background

AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

817206

UniProt

Q38998

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Ion metabolism

Dilution

1 : 50 with 125I (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

96.9 kDa

Precautions

In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)

Additionnal Information

For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1

References & Citations

Safiarian et al. (2015) . Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.Honsbein et al. (2009) . A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.