What is the catalog number for Anti-KC1 | potassium channel KAT3?

The catalog number for Anti-KC1 | potassium channel KAT3 is AS09 515.

What sizes are available for Anti-KC1 | potassium channel KAT3?

Anti-KC1 | potassium channel KAT3 is available in the following size(s): 200 µg.

Is Anti-KC1 | potassium channel KAT3 in stock?

Anti-KC1 | potassium channel KAT3 is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-KC1 | potassium channel KAT3

Product Specifications

Background

KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC

CAS Number

9007-83-4

HS Code

38221900

Gene ID

829400

UniProt

P92960

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Ion metabolism

Dilution

1 : 50 with 125I (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

75.5 | 75 kDa

Precautions

In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

Additionnal Information

For detection images please, refer to the publication belowAntibody detects native and recombinant KC1

References & Citations

Honsbein et al. (2009) . A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.