Anti-PEPC | Phosphoenolpyruvate carboxylase
Product Specifications
Background
PEPC(phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Host
Rabbit
Reactivity
Ananas comosus, Arabidopsis thaliana,Cenchrus ciliaris, Chenopodium quinoa, Chloris gayana, Chromera velia, Cyanthobasis fruticulosa, Glycine max, Hordeum vulgare, Jatropha curcas, Kochia prostrata, Leptochloa fusca, Lupinus sp. , Megathyrsus maximus, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Panicum antidotale, Panicum coloratum, Petrosimonia nigdeensis, Pinus strobus, Saccharumspp. hybrid clone C91-301, Salsola lanata, Salsola laricifolia,Salsola grandis, Salsola tragus Sorghum bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), Pinus strobus, Thalassiosira weissfloggi,Zea mays, Zostera muelleri
Not reactive in
Methanothermobacter thermautotrophicus
Immunogen
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species includingArabidopsis thalianaQ9MAH0,At1g53310(PEPC 1),Q84VW9,At3g14940(PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzymeNP_177043.2(PEPC 4).ForZea mays, the peptide is converved in PEP1 and PEP4 isoforms.
Clonality
Polyclonal
Applications
Immunolocalization (IL), Western blot (WB)
Dilution
1 : 500 (IL), 1: 1000 - : 10 000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
110 | 105 kDa
Precautions
Antibody can be also used following 2D gel electrophoresis
References & Citations
Fanget al. (2024). Subfunctionalisation and self-repression of duplicated E1 homologues finetunes soybean flowering and adaptation. Nat Commun. 2024 Jul 23;15(1):6184. doi: 10.1038/s41467-024-50623-3.Nguyenet al.(2024). The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs.J Integr Plant Biol. 2024 Jun 13.doi: 10.1111/jipb.13710.Cruzet al.(2023). Variation of photosynthesis along the canopy profile of sugarcane and energy canes. Research Square; DOI: 10.21203/rs.3.rs-3124093/v1Luo, et al. (2023) Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1. EMBO Rep. 2022;23(4):e53354. doi:10.15252/embr.202153355Dinget al. (2022) CPK28-NLP7 module integrates cold-induced Ca2+ signal and transcriptional reprogramming in Arabidopsis. Sci Adv. 2022 Jul;8(26):eabn7901. doi: 10.1126/sciadv.abn7901. Epub 2022 Jun 29. PMID: 35767615; PMCID: PMC9242591.Lan, Ma, Zheng, et al. (2022) Ubiquitome profiling reveals a regulatory pattern of UPL3 with UBP12 on metabolic-leaf senescence. Life Sci Alliance. 2022;5(12):e202201492. Published 2022 Aug 4. doi:10.26508/lsa.202201492Durallet al. (2021). Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt. Microb Cell Fact. 2021 Feb 8;20(1):39. doi: 10.1186/s12934-021-01529-y. PMID: 33557832; PMCID: PMC7871529.Wanget al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.Rakhmankulovaet al. (2021) Possible Activation of ?3 Photosynthesis in ?4 Halophyte Kochia prostrata Exposed to an Elevated Concentration of ??2. Russ J Plant Physiol 68, 1107–1114 (2021). https://doi.org/10.1134/S1021443721060169Durallet al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y.Krameret al. (2020). N6?methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis. Plant Direct . 2020 Jul 24;4(7):e00239.doi: 10.1002/pld3.239.Wanget al. (2019). PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15.Huiet al. (2018). TALE-carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice. Mol Plant Pathol. 2018 Nov 30. doi: 10.1111/mpp.12772.Salesse-Smithet al. (2018). Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018 Oct;4(10):802-810. doi: 10.1038/s41477-018-0252-4.Bassiet al. (2018). Nitrogen supply influences photosynthesis establishment along the sugarcane leaf. Sci Rep. 2018 Feb 2;8(1):2327. doi: 10.1038/s41598-018-20653-1.Sonawaneet al. (2018). Shade compromises the photosynthetic efficiency of NADP-ME less than PEP-CK and NAD-ME C 4 grasses. J Exp. Botany, doi.org/10.1093/jxb/ery129.Wenet al. (2017). Possible involvement of phosphoenolpyruvate carboxylase and NAD-malic enzyme in response to drought stress. A case study: A succulent nature of the C4-NAD-ME type desert plant, Salsola lanata (Chenopodiaceae). Functional Plant Biology 44(12), DOI10.1071/FP16430Jianget al. (2017). Development of an Efficient Protein Extraction Method Compatible with LC-MS/MS for Proteome Mapping in Two Australian Seagrasses Zostera muelleri and Posidonia australis. Frontiers in Plant Science, doi: 10.3389/fpls.2017.01416.Liuet al. (2017). Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response. Mol Cell. 2017 Apr 6;66(1):117-128.e5. doi: 10.1016/j.molcel.2017.02.016.Ribeiroet al. (2017). Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis. J Plant Physiol. 2017 Jan;208:61-69. doi: 10.1016/j.jplph.2016.11.005.Shenet al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.Ishikawaet al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print]Shenet al. (2015). Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane. Photosynthetica, September 2015, Volume 53, Issue 3, pp 436-446.Foleyet. al (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biology 2015, 15:106 doi:10.1186/s12870-015-0485-6.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6703d4/datasheet/as09 458.pdf
CAS Number
9007-83-4
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items