Anti-AO | ascorbate oxidase
Product Specifications
Background
Ascorbate oxidase (AO)is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Host
Rabbit
Reactivity
Cucurbitasp.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Ascorbate oxidase purified fromCucurbitasp.
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Dilution
1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Purity
Total IgG in PBS pH 7.2.
Format
Lyophilized
Reconstitution
For reconstitution add 0.5 ml of sterile water
Molecular Weight
61 | 45 kDa (Cucurbita pepo)
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6701c3/datasheet/as09 384.pdf
CAS Number
9007-83-4
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