Anti-AO | ascorbate oxidase

Product Specifications

Background

Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA) . Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Cucurbita sp.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Ascorbate oxidase purified from Cucurbita sp.

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

Plant Oxidative Stress

Dilution

1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Purity

Total IgG in PBS pH 7.2.

Format

Lyophilized

Reconstitution

For reconstitution add 0.5 ml of sterile water

Molecular Weight

61 | 45 kDa (Cucurbita pepo)

Additionnal Information

IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.