Anti-APX | L-ascorbate peroxidase
Product Specifications
Background
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX) .
CAS Number
9007-83-4
HS Code
38221900
Gene ID
844085
UniProt
Q42593 , Q42592 , Q05431 , Q1PER6 , Q42564
Host
Rabbit
Reactivity
Arabidopsis thaliana, Armeria maritima, Brassica napus, Capsicum annuum, Citrus sp., Digitaria sanguinalis, Dionaea muscipula, Echinochla crus-galli, Iris pumila, Lathyrus sativus, Liquidambar formosana, Lupin sp., Manihot esculenta, Medicago sativa, Nicotiana tabacum thylakoid-bound APX, stromal APX; Oryza sativa, Panicum milaceum, Plumbago zeylanica, Schima superba, Silene vulgaris, Solanum lycopersicum, Spinacia oleracea (stromal APX, thylakoid-bound), Triticum aestivum
Not reactive in
Algae, Helianthus annus, Marchantia polymorpha
Immunogen
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1, TAIR:At1g77490 and sAPX (stromal/mitochondrial ascorbate peroxidase) UniProt: Q42592-1 TAIR: At4g08390 Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cAPX2 (At3g09640) and pAPX (At4g35000)
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Plant Oxidative Stress
Dilution
1 : 2000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
25-38 kDa for A. thaliana
Precautions
This product can be sold containing proclin if requested
References & Citations
Kolbert et al. (2023) . Nitro-oxidative response to internalized multi-walled carbon nanotubes in Brassica napus and Solanum lycopersicum. Ecotoxicol Environ Saf. 2023 Nov 15:267:115633. doi: 10.1016/j.ecoenv.2023.115633. Bismarck et al. (2023) . Growth in fluctuating light buffers plants against photorespiratory perturbations. Nat Commun . 2023 Nov 3;14 (1) :7052. doi: 10.1038/s41467-023-42648-x.Wang et al. (2022) Reciprocity between a retrograde signal and a putative metalloprotease reconfigures plastidial metabolic and structural states. Sci Adv. 2022 Jun 3;8 (22) :eabo0724. doi: 10.1126/sciadv.abo0724. Epub 2022 Jun 3. PMID: 35658042; PMCID: PMC9166295.Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. (https://www.sciencedirect.com/science/article/pii/S0168945221003691) Jedelska et al. (2021) Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp. Hortic Res. 2021 Feb 1;8 (1) :34. doi: 10.1038/s41438-021-00469-3. PMID: 33518717; PMCID: PMC7848004.Tokarz et al. (2021) . Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22 (2) :685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Tokarz et al. (2020) . Can Ceylon Leadwort (Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21 (5) :1866.doi: 10.3390/ijms21051866.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Brassica rapa subsp. oleifera Stromal APX; Glycine max, Glycine soja L-ascorbate peroxidase T, chloroplastic; Medicago truncatula thylakoid-bound APX; Mesembryanthemum crystallinum, Pisum sativum Chloroplast stromal ascorbate peroxidase 12; Solanum lycopersicum thylakoid-bound APX; Spinacia oleracea stromal APX; Theobroma cacao L-APX T isoform 3; Vitis viniferaSpecies of your interest not listed? Contact us
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