Anti-Cyt c | Cytochrome c
Product Specifications
Background
Cytochrome cis located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
Host
Rabbit
Reactivity
Arabidopsis thaliana, Brassica oleracea, Glycine max, Pisum sativum, Zea mays
Not reactive in
Arabidopsis thalianaCytC6,Chinesecuscutasp.
Immunogen
KLH-conjugated synthetic peptide derived fromArabidopsis thalianacytochrome c protein sequence, UniProt:D7KMK0(C-1)D7LY03(C-2), TAIR:At1g22840(Cytc1) andAt4g10040(Cytc2)
Clonality
Polyclonal
Applications
Immunolocalization (IL), Western blot (WB)
Dilution
1: 100 (IL), 1 : 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
12.5 | 14 kDa (forArabidopsis thaliana)
Precautions
https://www.agrisera.com/en/artiklar/goat-anti-rabbit-igg-hl-ap-conjugated-.htmlThe presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death)
References & Citations
Chenet al. (2023) The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC.Nguyenet al. (2022). MISF2 Encodes an Essential Mitochondrial Splicing Cofactor Required for nad2 mRNA Processing and Embryo Development in Arabidopsis thaliana. Int J Mol Sci. 2022 Feb 28;23(5):2670. doi: 10.3390/ijms23052670. PMID: 35269810; PMCID: PMC8910670.Soriaet al. (2024).Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Canalet al. (2024).Cytochrome c levels affect the TOR pathway to regulate growth and metabolism under energy-deficient conditions. New Phytol. 2024 Mar;241(5):2039-2058.Guoet al. (2021) The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana,Biochemical and Biophysical Research Communications,Volume 578,2021,Pages 63-69,ISSN 0006-291X,https://doi.org/10.1016/j.bbrc.2021.09.022.(https://www.sciencedirect.com/science/article/pii/S0006291X21013164)Wanget al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Daiet al. (2020). Pentatricopeptide repeat protein DEK46 is required for multi-sites mitochondrial RNA editing and maize seed development. J Exp Bot. 2020 Jul 25;eraa348.doi: 10.1093/jxb/eraa348.Waltzet al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Doroninaet al. (2019). Structural and Functional Features of the Wheat Embryo Sac’s Antipodal Cells during Differentiation. Russ J Dev Biol 50, 194–208. (immunolocalization).Rureket al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Daiet al. (2018). Maize Dek37 Encodes a P-Type PPR Protein That Affects Cis-splicing of Mitochondrial nad2 Intron 1 and Seed Development. Genetics. 2018 Jan 4. pii: genetics.300602.2017. doi: 10.1534/genetics.117.300602.Opalinskaet al. (2017). Identification of Physiological Substrates and Binding Partners of the Plant Mitochondrial Protease FTSH4 by the Trapping Approach. Int J Mol Sci. 2017 Nov 18;18(11). pii: E2455. doi: 10.3390/ijms18112455.Schimmeyeret al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Liet al. (2016). Characterization of a novel Beta-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. J Exp Bot. 2016 Nov;67(21):6061-6075. Epub 2016 Oct 6.
Storage Conditions
Store lyophilized at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc67039d/datasheet/as08 343a.pdf
CAS Number
9007-83-4
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