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Anti-VRN2 | Vernalization protein 2

Product Specifications

Background

Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

Triticum aestivum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Clonality

Monoclonal

Applications

ELISA (ELISA), Western blot (WB)

Field of Research

Photomorphogenesis

Dilution

1 : 10 000 (ELISA), 1 : 1000 (WB)

Purity

Total IgG. Protein G purified purified from Cell culture supernatant.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

23.7 kDa

Precautions

There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum

Additionnal Information

VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Triticum monococcumSpecies of your interest not listed? Contact us

Frequently Asked Questions

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