Anti-VRN2 | Vernalization protein 2
Product Specifications
Background
Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
Triticum aestivum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
Clonality
Monoclonal
Applications
ELISA (ELISA), Western blot (WB)
Field of Research
Photomorphogenesis
Dilution
1 : 10 000 (ELISA), 1 : 1000 (WB)
Purity
Total IgG. Protein G purified purified from Cell culture supernatant.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
23.7 kDa
Precautions
There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum
Additionnal Information
VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Triticum monococcumSpecies of your interest not listed? Contact us
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