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Anti-AtpC | Gamma subunit of ATP synthase (chloroplastic)

Product Specifications

Background

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF (1) - the catalytic core - and CF (0) - the membrane proton channel. CF (1) has five subunits: alpha (3), beta (3), gamma (1), delta (1), epsilon (1) . CF (0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF (0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

825797

UniProt

Q01908 , Q01909

Host

Rabbit

Reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sorokiniana, Chlorella vulgaris, Echinochloa crus-galli, Phycomitrella patens, Pisum sativum, Zea mays

Not reactive in

Phaeodactylum tricornutum

Immunogen

KLH-conjugated peptide, derived from C-terminal part of Chlamydomonas reinhardtii protein sequence A8HXL8

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

ATP synthase

Dilution

1: 1000 (ELISA), 1: 10 000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

35.3 | 42 (Chlamydomonas reinhardtii) 35.6 | 38 (Spinacia oleracea)

Precautions

Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure inLemaireet al. (1989).

Additionnal Information

This product can be sold containing ProClin if requested

References & Citations

Kafri et al. (2023) . Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186 (25) :5638-5655.e25.doi: 10.1016/j.cell.2023.11.007. Pipitone et al. (2021) . A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Storti et al. (2020) . The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Pralon et al. (2019) . Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.Li et al. (2019) . A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51 (4) :627-635. doi: 10.1038/s41588-019-0370-6.Liang et al. (2018) . Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30 (7) :1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Arachis hypogaea, Auxenochlorella protothecoides, Bathycoccus prasinos, Cephalotus follicularis, Cicer arietinum, Coccomyxa subellipsoidea (strain C-169), Cucumis melo, Cyanobacteria, Cynara cardunculus var. scolymus, Daucus carota subsp. sativus, Dendrobium catenatum, Fagus sylvaticaGenlisea aurea, Glycine max, Gossypium hirsutum, Jatropha curcas, Juglans regia, Klebsormidium flaccidum, Helianthus annuus, Lactuca sativa, Lens culinaris, Lupinus angustifolius, Manichot esculenta, Marchantia polymorpha subsp. ruderalis, Medicago truncatula, Micromonas pusilla (strain CCMP1545), Monoraphidium neglectum, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Ostreococcus tauri, Punica granatum , Phaseolus vulgaris, Pisum sativum, Populus jackii, Populus trichocarpa, Prunus persica, Ricinus communis, Rosa chinensis, Selaginella moellendorffii, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Terma orientalis, Tetraselmis sp. GSL018, Theobroma cacao, Trifolium pratense, Zostera marina, Vigna unguiculata, Vitis vinifera, Volvox carteri f. nagariensis, Quercus suber Species of your interest not listed? Contact us
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