Anti-NR | Nitrate reductase, assimilatory

Assimilatory nitrate reductase (NR), (EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD (P) H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level) . NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3) .

Arabidopsis thaliana, Chlamydomonas reinardtii, red alga Gracilaria gracilis, Hordeum vulgare, Leptodictyum riparium (Hedw.) Warnst (moss), Medicago sativa, Phaeodactylum tricornutum Bohlin accession Pt1 8.6, Panax notoginseng, Populus yunanensis Dode, Solanum lycopersicum, Solanum tuberosum, Thalassiosira sp. (diatom), Trebouxia sp., Vigna radiata, Vitis vinigera

KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130

InChlamydmonas reinhardtiianti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).Using this antibody genome editing inChlorella vulgarisUTEX395 by CRISPR-Cas9 system has been demonstrated as described inKimet al. (2021)Chemiluminescent detection is advised for NR detection using this antibody.

Expósito et al. (2023) . Symbiotic interactions in the lichen R. farinacea dramatically modify NO biosynthetic source in Trebouxia microalgae.Algal Research Volume 75, September 2023, 103247.Costa-Broseta et al. (2021) . Post-Translational Modifications of Nitrate Reductases Autoregulates Nitric Oxide Biosynthesis in Arabidopsis. Int J Mol Sci. 2021 Jan 7;22 (2) :E549. doi: 10.3390/ijms22020549. PMID: 33430433.Kim et al. (2021) . Establishment of a Genome Editing Tool Using CRISPR-Cas9 in Chlorella vulgaris UTEX395. Int J Mol Sci. 2021 Jan 6;22 (2) :E480. doi: 10.3390/ijms22020480. PMID: 33418923.Prinsi et al. (2021) . Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability. Plants 10, no. 4: 792. https://doi.org/10.3390/plants10040792Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Zhang et al. (2020) . Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020 Feb 3. doi: 10.1111/pce.13736.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Arabis alpina, Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Citrus sinensis, Chlorella vulgaris, Dunaliella salina, marine Diatoms, Coffea canephora, Eucalyptus grandis, Glycine max, Glycine soja, Gossypium arboretum, Helianthus annuus, Lycopersicum esculentum, Morus alba, Nannochloropsis gaditana, Nicotiana tabacum, Nicotiana attenuata, Nicotiana benthamiana, Oryza sativa, Phaseolus vulgaris, Phytophthora infestans, Physcomitrium patens, Prunus persica, Ricinus communis, Sorghum bicolor, Spinacia oleracea, Solanum lycopersicum, Symbiodinium microadriaticum, Theobroma cacao, Zea maysSpecies of your interest not listed? Contact us