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Anti-HSP17,7 | Cytosolic class II heat shock protein 17,7

Product Specifications

Background

Hsp17.7belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Host

Rabbit

Reactivity

Arabidopsis thaliana, Agave tequilianavar. Weber, Cucumis sativus, Medicago sativa, Pinellia ternata, Silene vulgaris

Not reactive in

Oryza sativam, Polyscias elegans

Immunogen

Full-length recombinant protein produced inE. coliand purified by conventional methods (no affinity tag).Arabidopsis thalianaHsp17.7 CII (class two), UniProt:O81822, TAIR:At5g12030

Clonality

Polyclonal

Applications

Western blot (WB)

Dilution

1 : 1000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

17.7 kDa

References & Citations

Flores-Cácereset al. (2023). The Early Oxidative Stress Induced by Mercury and Cadmium Is Modulated by Ethylene in Medicago sativa Seedlings. Antioxidants 2023, 12, 551. https://doi.org/10.3390/antiox12030551Chaet al. (2020). Humic acid enhances heat stress tolerance via transcriptional activation of Heat-Shock Proteins in Arabidopsis. Sci Rep. 2020 Sep 14;10(1):15042.doi: 10.1038/s41598-020-71701-8.McLoughlinet al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263Fuet al. (2019). Increased fes1a thermotolerance is induced by BAG6 knockout. Plant Mol Biol. 2019 Feb 22. doi: 10.1007/s11103-019-00844-8.Korotaevaet al. (2018). Effect of Heat Hardening on Expression of Genes phb3 and phb4 and Accumulation of Phb Proteins in Green Leaves of Arabidopsis thaliana. Russian Journal of Plant Physiology, 65(5), 688-696, 2018. https://doi.org/10.1134/s1021443718040039McLoughlinet al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Hattabet al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.Maoand Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.Janicka-Russaket al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.Lujanet al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6701aa/datasheet/as07 255.pdf

CAS Number

9007-83-4

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