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Anti-PEPCK | PEP carboxykinase

Product Specifications

Background

Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

541622

UniProt

Q9SLZ0

Host

Rabbit

Reactivity

Ananas comosus, Miscantus giganteus, Mouse, Nannochloropsis oceanica, Oryza sativa, Panicum maximum, , Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301, Setaria viridis, Spartina alterniflora, Spartina patens, Zea mays

Not reactive in

Arabidopsis thaliana, Pinus yunnanensis

Immunogen

KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Global Antibodies

Dilution

1 : 1000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Molecular Weight

73 | 78 kDa

Precautions

Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.Antibody can be also used following 2D gel electrophoresis.This product can be sold containing ProClin if requested.

References & Citations

Wei et al. (2019) . Transcriptomic and proteomic responses to very low ­CO 2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica. Biotechnol Biofuels 12: 168.Shen et al. (2016) . The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y) . 2016 Dec;9 (1) :20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.Aragón et al. (2013) . The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Brassica napus, Chromera velia, Cucumis sativus, Flaveria sp., Lycopersicon esculentum, Medicago sativa, Oryza sativa, Urochloa panicoides, Zoysia japonica, Zea mays Species of your interest not listed? Contact us

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