Anti-PEPCK | PEP carboxykinase
Product Specifications
Background
Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
541622
UniProt
Q9SLZ0
Host
Rabbit
Reactivity
Ananas comosus, Miscantus giganteus, Mouse, Nannochloropsis oceanica, Oryza sativa, Panicum maximum, , Panicum virgatum, Phaseolus vulgaris, Saccharum spp. hybrid clone C91-301, Setaria viridis, Spartina alterniflora, Spartina patens, Zea mays
Not reactive in
Arabidopsis thaliana, Pinus yunnanensis
Immunogen
KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Global Antibodies
Dilution
1 : 1000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Molecular Weight
73 | 78 kDa
Precautions
Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.Antibody can be also used following 2D gel electrophoresis.This product can be sold containing ProClin if requested.
References & Citations
Wei et al. (2019) . Transcriptomic and proteomic responses to very low CO 2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica. Biotechnol Biofuels 12: 168.Shen et al. (2016) . The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y) . 2016 Dec;9 (1) :20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.Aragón et al. (2013) . The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Brassica napus, Chromera velia, Cucumis sativus, Flaveria sp., Lycopersicon esculentum, Medicago sativa, Oryza sativa, Urochloa panicoides, Zoysia japonica, Zea mays Species of your interest not listed? Contact us
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