Anti-PhyA | Phytochrome A
Product Specifications
Background
Phytochrome A (PhyA)is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.
Host
Rabbit
Reactivity
Arabidopsis thaliana, Arachis hypogaeaL., Nicotiana benthamiana, Nicotiana tabacum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences includingArabidopsis thalianaUniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
124 kDa
Precautions
Careful sample collection is adviced to assure the best results with this antibody
References & Citations
Staudtet al. (2023). EID1 promotes the response to canopy shade in Arabidopsis thaliana by repressing the action of phytochrome A. MicroPubl Biol. 2023 Dec 12:2023:10.17912/micropub.biology.001015.doi: 10.17912/micropub.biology.001015.Schwenket al. (2021) Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants. Elife. 2021 Mar 30;10:e63697. doi: 10.7554/eLife.63697. PMID: 33783355; PMCID: PMC8009681.Schenket al. (2021) Light-induced degradation of SPA2 via its N-terminal kinase domain is required for photomorphogenesis, Plant Physiology, 2021;, kiab156, https://doi.org/10.1093/plphys/kiab156Menonet al. (2019). Arabidopsis FAR-RED ELONGATED HYPOCOTYL 1 and FHY1-LIKE are not required for phytochrome A signal transduction in the nucleus. Plant Communications. Available online 9 November 2019, 100007.Agliassaet al. (2018). Geomagnetic field impacts on cryptochrome and phytochrome signaling. J Photochem Photobiol B. 2018 Aug;185:32-40. doi: 10.1016/j.jphotobiol.2018.05.027.Zhanget al. (2018). Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13(5):e0198041. doi: 10.1371/journal.pone.0198041.Pierto-Dapenaet al. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. J Exp Bot. 2017 Feb 16. doi: 10.1093/jxb/erx020.Paiket al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6702c8/datasheet/as07 220.pdf
CAS Number
9007-83-4
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