Anti-PhyA | Phytochrome A

Product Specifications

Background

Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

837483

UniProt

P14712

Host

Rabbit

Reactivity

Arabidopsis thaliana, Arachis hypogaea L., Nicotiana benthamiana, Nicotiana tabacum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Photomorphogenesis

Dilution

1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

124 kDa

Precautions

Careful sample collection is adviced to assure the best results with this antibody

Additionnal Information

In vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in Paik et al. (2012) . Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4) : 1335-1340.

References & Citations

Staudt et al. (2023) . EID1 promotes the response to canopy shade in Arabidopsis thaliana by repressing the action of phytochrome A. MicroPubl Biol. 2023 Dec 12:2023:10.17912/micropub.biology.001015.doi: 10.17912/micropub.biology.001015. Schwenk et al. (2021) Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants. Elife. 2021 Mar 30;10:e63697. doi: 10.7554/eLife.63697. PMID: 33783355; PMCID: PMC8009681.Schenk et al. (2021) Light-induced degradation of SPA2 via its N-terminal kinase domain is required for photomorphogenesis, Plant Physiology, 2021; , kiab156, https://doi.org/10.1093/plphys/kiab156Menon et al. (2019) . Arabidopsis FAR-RED ELONGATED HYPOCOTYL 1 and FHY1-LIKE are not required for phytochrome A signal transduction in the nucleus. Plant Communications. Available online 9 November 2019, 100007.Agliassa et al. (2018) . Geomagnetic field impacts on cryptochrome and phytochrome signaling. J Photochem Photobiol B. 2018 Aug;185:32-40. doi: 10.1016/j.jphotobiol.2018.05.027.Zhang et al. (2018) . Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13 (5) :e0198041. doi: 10.1371/journal.pone.0198041.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Brassica rapa, Cardamine hirsuta, Dacucus carota, Lathyrus sativus, Fragaria ananassa, Glycine max, Gossyoium hirsutum, Hordeum vulgare, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum lycopersicumSpecies of your interest not listed? Contact us