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Anti-Rubisco | 557 kDa hexadecamer

Product Specifications

Background

Rubisco(Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Host

Rabbit

Reactivity

Anabaenasp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculentaCrantz, Oryza sativa, Physcomitrium patens, Pisum sativum,Populussp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea,Synechocystissp. PCC 6803, Synechococcussp. PCC7942, Zea mays

Not reactive in

Chlamydomonas reinhardtii(immulolocalization)

Immunogen

purified 557 kDa hexadecamer Rubisco protein complex fromSpinacia oleracea(SIGMA-ALDRICHR-8000), UniProt:P00875andQ43832

Clonality

Polyclonal

Applications

Immunolocalization (IL), Western blot (WB)

Dilution

1 : 10 000-1 : 20 000 on 0,5-10 ug total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

53-55 | 53-55 kDa

Precautions

RbcS subunit is not detected by this antibodyThis product can be sold containing proclin if requested

References & Citations

Miharaet al. (2019). Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner. J Exp Bot. 2019 Dec 21. pii: erz561. doi: 10.1093/jxb/erz561.Sedaghatmehret al. (2019). A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. 2019 Mar;42(3):1054-1064. doi: 10.1111/pce.13426.Rohnkeet al. (2018). RcaE-Dependent Regulation of Carboxysome Structural Proteins Has a Central Role in Environmental Determination of Carboxysome Morphology and Abundance in Fremyella diplosiphon. Mol Biol and Physiol. Vol. 3, Issue 1. DOI: 10.1128/mSphere.00617-17Zhanget al. (2017). Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.Wanget al. (2017). Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27(21):3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.Wenand Zhang (2014). Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae). Photosynth Res. 2014 Sep 17. (immunolocalization)Feifeiet al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70a994c49e6dc66f91f/datasheet/as07 218.pdf

CAS Number

9007-83-4

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