Anti-Rubisco | 557 kDa hexadecamer

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays

Mihara et al. (2019) . Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner. J Exp Bot. 2019 Dec 21. pii: erz561. doi: 10.1093/jxb/erz561.Sedaghatmehr et al. (2019) . A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. 2019 Mar;42 (3) :1054-1064. doi: 10.1111/pce.13426.Rohnke et al. (2018) . RcaE-Dependent Regulation of Carboxysome Structural Proteins Has a Central Role in Environmental Determination of Carboxysome Morphology and Abundance in Fremyella diplosiphon. Mol Biol and Physiol. Vol. 3, Issue 1. DOI: 10.1128/mSphere.00617-17Zhang et al. (2017) . Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.Wang et al. (2017) . Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27 (21) :3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.Wen and Zhang (2014) . Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae) . Photosynth Res. 2014 Sep 17. (immunolocalization) Feifei et al. (2014) . Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9 (4) :e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.