Anti-PR-3 / CHN | Class I chitinase

Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known.  Glucanase I  (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Agostis stoloniferacv. ‘Penncross’,Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992): 1) Chitinase A (CHN A)P08252encoded by gene chn48 derived from theN. tomentosiformisancestor of tobacco. 2) Chitinase B (CHN B)P24091encoded by gene chn50 derived from theN. sylvestrisancestor of tobacco.

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Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.