Anti-PR-3 / CHN | Class I chitinase

Product Specifications

Background

Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

107759005

UniProt

P08252 , P24091

Host

Rabbit

Reactivity

Agostis stolonifera cv. ‘Penncross’, Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

Not reactive in

Cicer arietinum

Immunogen

Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992) : 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.

Clonality

Polyclonal

Applications

Co-Immunoprecipitation (IP) (Co-IP), Immunolocalization (IL), Western blot (WB)

Field of Research

Pathogen attack

Dilution

8 µg/ml (WB)

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

35, 34 | 32 and 34 kDa

Precautions

Important note: For blocking 5% skim milk in PBS without Ca++ should be used. This antibody is purified by affinity chromarography on Portein G.

Additionnal Information

Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa) This antibody can be used as a marker of vacuolar contents Keefe et al. (1990) . The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

References & Citations

Mansilla et al. (2020) .- Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants. Eur J Plant Pathol 158, 211?222 (2020) . https://doi.org/10.1007/s10658-020-02067-wColman et al. (2019) . Chitosan microparticles improve tomato seedling biomass and modulate hormonal, redox and defense pathways. Plant Physiology and Biochemistry Volume 143, October 2019, Pages 203-211. Kumari et al. (2017), Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis. Front Plant Sci. 2017 Jan 6;7:2027. doi: 10.3389/fpls.2016.02027.Jespersen et al. (2017) . Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ?Penncross?) Ko et al. (2016) . Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244 (2) :379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.