Anti-IDH | Isocitrate dehydrogenase (Cellular [compartment marker] of mitochondrial matrix)

Plant NADH dependent isocitrate dehydrogenase enzyme is located in mitochondrial matrix. This enzyme is classified as an oxidoreductase and its function is to catalyze a reaction in the citric acid cycle, specifically the sequential dehydrogenation and decarboxylation of isocitrate to form a-ketoglutarate. It removes hydrogens from its substrate, isocitrate. In addition to this process, it functions as a decarboxylase, removing a CO2 from the six-carbon substrate to form a five-carbon product mentioned above as a-ketoglutarate. There are two forms of this enzyme NADP+ and NAD+ dependent.

Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Glycine max, Lycopersicum chilense, Nicotiana benthamiana, Oryza sativa, Solanum lycopersicum, Pisum sativum, Solanum sogarandium, Solanum tuberosum, Zea mays

KLH-conjugated peptide 1 and peptide 2 conserved in all higher plants mitochondrial, NAD dependent isocitrate dehydrogenase subunits including Arabidopsis thaliana IDH-I Q8LFC0, At4g35260 and IDH-II P93032, At2g17130

Peptide used to elicit this antibody is not conserved in NADPH dependent anzymes, partially conserved across eukaryotic Idh subunits, Some conservation across bacterial which contain the NAD-dependent form of Idh (as opposed to the NADP-dependent form)

Boussardon et al. (2025) . The atypical proteome of mitochondria from mature pollen grains. Curr Biol . 2025 Jan 21:S0960-9822 (24) 01705-6. doi: 10.1016/j.cub.2024.12.037. Hoa et al. (2024) . Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules. Comparative Study Plant Cell Physiol. 2004 Mar;45 (3) :300-8. doi: 10.1093/pcp/pch035. Boussardon et al. (2023) . Comparing plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells. J Exp Bot. 2023 Apr 7:erad133. doi: 10.1093/jxb/erad133.Li et al. (2022) The CDC48 complex mediates ubiquitin-dependent degradation of intra-chloroplast proteins in plants. Cell Rep. 2022 Apr 12;39 (2) :110664. doi: 10.1016/j.celrep.2022.110664. PMID: 35417702.Kolodziejczak et al. (2018) . m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59 (5) :1006-1016. doi: 10.1093/pcp/pcy041.Rurek et al. (2018) . Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1) .Fujii et al. (2016) . The Restorer-of-fertility-like 2 pentatricopeptide repeat protein and RNase P are required for the processing of mitochondrial orf291 RNA in Arabidopsis. Plant J. 2016 Jun;86 (6) :504-13. doi: 10.1111/tpj.13185.Yin et al. (2016) . Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11 (4) :e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Rurek et al. (2015) . Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728 (15) 00016-X. doi: 10.1016/j.bbabio.2015.01.005.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Brachypodium distachyon, Brassica napus, Capsella rubella, Citrus sinensis, Hordeum vulgare, Malus x domestica, Medicago truncatula, Nicotiana tabacum, Phaseolus vulgaris, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays Species of your interest not listed? Contact us