Anti-GS | Glutathione synthase, GSH-S

Product Specifications

Background

Glutathione synthetase (GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS) ; in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development

CAS Number

9007-83-4

HS Code

38221900

Gene ID

832797

UniProt

Q8W4W2

Host

Rabbit

Reactivity

Brassica campestris, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum, Solanum lycopersicum, Zea mays

Not reactive in

Galdieria sulphuraria

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays GSH-S sequence Q8W4W2

Clonality

Polyclonal

Applications

Immunolocalization (IL), Western blot (WB)

Field of Research

Plant Oxidative Stress

Dilution

1 : 2000 (WB)

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

45 kDa

Precautions

Immunolocalization has been done forArabidopsis thalianaandNicotiana tabacum

Additionnal Information

Total IgG concentration is 3,7 µg/µl

References & Citations

Sun et a. (2019) . Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. Int J Mol Sci. 2019 Dec 26;21 (1) . pii: E180. doi: 10.3390/ijms21010180.Jayawardena et al. (2016) . Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]Baojian et al. (2014) . Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines. Proteomics, DOI: 10.1002/pmic.201300147.Gomez et al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Aegilops tauschii, Arabidopsis thaliana, Arundo donax, Asparagus officinalis, Brachypodium distachyon, Capsicum annuum, Cicer arietinum, Citrus clementina, Corchorus olitorius, Cucumis sativus, Daucus carota subsp. sativus, Dimocarpus longan, Eucalyptus grandis, Gossypium hirsutum , Helianthus annuus , Hevea brasiliensis, Hordeum vulgare, Jatropha curcas, Lotus japonicus, Lupinus angustifolius, Lycium chinense, Musa acuminata, Nelumbo nucifera, Nicotiana tabacum, Nicotiana sylvestris, Pisum sativum, Setaria italica, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Zostera marina, Vitis vinifera Species of your interest not listed? Contact us