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Anti-GS | Glutathione synthase, GSH-S

Product Specifications

Background

Glutathione synthetase(GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS); in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development

Host

Rabbit

Reactivity

Brassica campestris, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum, Solanum lycopersicum, Zea mays

Not reactive in

Galdieria sulphuraria

Immunogen

KLH-conjugated synthetic peptide derived fromZea maysGSH-S sequenceQ8W4W2

Clonality

Polyclonal

Applications

Immunolocalization (IL), Western blot (WB)

Dilution

1 : 2000 (WB)

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

45 kDa

Precautions

Immunolocalization has been done forArabidopsis thalianaandNicotiana tabacum

References & Citations

Sunet a. (2019). Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. Int J Mol Sci. 2019 Dec 26;21(1). pii: E180. doi: 10.3390/ijms21010180.Jayawardenaet al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]Baojianet al. (2014). Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines. Proteomics, DOI: 10.1002/pmic.201300147.Gomezet al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc67041e/datasheet/as06 183.pdf

CAS Number

9007-83-4

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