Anti-cAPX | Ascorbate peroxidase (cytosolic) (plant)
Product Specifications
Background
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.
CAS Number
9007-83-4
Host
Rabbit
Reactivity
Arabidopsis thaliana,A. toxicaria,Hordeum vulgare, Medicago sativa, Nicotiana tabacum, Oryza sativa, Pisum sativum, Salicorniasp., Silene vulgaris, Solanum lycopersicum, Solanum lycopersicum, Solanum tuberosum, Picea silvestris, Zea mays, Zygophyllum fabago
Not reactive in
Algae, Galdieria sulphuraria, Glycine max
Immunogen
KLH-conjugated peptide derived from N-terminal ofZea mayscytosolic APX UniProt:Q41772
Clonality
Polyclonal
Applications
Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB)
Dilution
2 µg (IP), 1 : 500 (IL), 1: 2000 - 1 : 10 000 (WB)
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Molecular Weight
28 kDa
References & Citations
Liet al. (2024). An NLR paralog Pit2 generated from tandem duplication of Pit1 fine-tunes Pit1 localization and function. Nat Commun. 2024 May 30;15(1):4610. doi: 10.1038/s41467-024-48943-5.Flores-Cácereset al. (2023). The Early Oxidative Stress Induced by Mercury and Cadmium Is Modulated by Ethylene in Medicago sativa Seedlings. Antioxidants 2023, 12, 551. https://doi.org/10.3390/antiox12030551Bègueet al. (2019). CDC48 regulates ascorbate peroxidase in tobacco. J Exp Bot. 2019 Mar 1. pii: erz097. doi: 10.1093/jxb/erz097.Wanget al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115.Balážováet al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Adhikariet al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Ferreret al. (2018). Differential Pb tolerance in metallicolous and non-metallicolous Zygophyllum fabago populations involves the strengthening of the antioxidative pathways. Environ & Exp Botany, Vo. 150, June 2018, Pages 141-151.Arocaet al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015.Terrileet al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814.Tsaniklidiset al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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