Anti-CSD2 | Chloroplastic Cu/Zn superoxide dismutase

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202, . SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Arabidopsis thaliana, Armeria maritima, Brassica juncea, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Hordeum vulgare, Impatiens walleriana, Iris pumila, Liquidambar formosana, Medicago sativa, Musa acuminate, Musa paradisiaca L., Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays

Overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.

Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form

Böde et al. (2025) . Lipid Phase Behaviour of the Curvature Region of Thylakoid Membranes of Spinacia oleracea. Physiol Plant. 2025 May-Jun;177 (3) :e70289. doi: 10.1111/ppl.70289. Krupinska et al. (2025) . Iron allocation to chloroplast proteins depends on the DNA-binding protein WHIRLY1. Planta. 2025 Jun 17;262 (2) :32. doi: 10.1007/s00425-025-04736-8. Zhuang et al (2021) . EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis. Cell Rep. 2021 Jul 13;36 (2) :109384. doi: 10.1016/j.celrep.2021.109384. PMID: 34260941.Wang et al. (2021) . Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.Fesharaki-Esfahani et al. (2021) A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina, Phytochemistry, Volume 187,2021,112766, ISSN 0031-9422, https://doi.org/10.1016/j.phytochem.2021.112766.Konkolewska et al. (2020) . Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals (Zn, Pb, Cd) . Shinozaki et al. (2020) . Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.