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Anti-NtrC | Thioredoxin reductase (TR/TRxR) (algal)

Product Specifications

Background

Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx) . Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

5715808

UniProt

A8HNQ7

Host

Rabbit

Reactivity

Chlamydomonas reinhardtii

Not reactive in

Arabidopsis thaliana

Immunogen

KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Chlamydomonas reinhardtii

Dilution

1 : 500 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

55.3 | 49 kDa (following processing)

Precautions

Antibody will react with long NtrC ofChlamydomonas reinhardtiiproteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.

Additionnal Information

Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Chlamydia sp., Marchantia polymorpha, Nannochloropsis gaditana, Ostreococcus luminarius, Physcomitrium patens, Synechococcus sp.Species of your interest not listed? Contact us

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