Anti-AOX1 | Algal Alternative oxidase 1
Product Specifications
Background
The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
5720157
UniProt
O65000
Host
Rabbit
Reactivity
Chlamydomonas reinhardtii
Not reactive in
Cyanidioschyzon merolae
Immunogen
Whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Mitochondria | Respiration
Dilution
1 : 10 000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
36 | 36 kDa (Chlamydomonas reainhardii)
Additionnal Information
Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane
References & Citations
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022) . https://doi.org/10.1038/s41586-022-04662-9Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiPerlaza et al. (2019) . The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.Kaye et al. (2019) . The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light.J Biol Chem. 2019 Jan 25;294 (4) :1380-1395. doi: 10.1074/jbc.RA118.004667.Zalutskaya et al. (2015) . The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Nannochloropsis gaditana, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis Species of your interest not listed? Contact us
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