Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Load per well on cell extract ofPinus banksiana(Jack Pine) was 7 µg.This antibody can be used as a loading control forChlamydomonas reinhardtiiwhile it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schultet al. 2007).

Collombat et al. (2025) . Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis. Commun Biol . 2025 Jan 22;8 (1) :111. doi: 10.1038/s42003-025-07497-y.Mu et al. (2024) . Plastid HSP90C C-terminal extension region plays a regulatory role in chaperone activity and client binding.Plant J. 2024 Jul 5.doi: 10.1111/tpj.16917. Lim et al (2022) . Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13 (1) :652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Lim et al (2022) Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13 (1) :652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Jiang et al. (2020) . Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27; eraa399.doi: 10.1093/jxb/eraa399. Nama et al. (2018) . Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.