Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit) (10 µl)
Product Specifications
Background
ATP synthaseis the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Host
Rabbit
Reactivity
Agostis stoloniferacv. ‘Penncross’, Arabidopsis thaliana, Bacillus cereus, Chlamydomonas reinhardtii, Hordeum vulgare, Glycine max, Lycopersicum esulentum, Oryza sp.(roots, leafs, pollen),Nicotiana bentamiana, Nicotiana tabacum,Populus sp., Spinacia oleracea, Zea mays. Animal tissues from: cow, chicken, pig, rat, salmon, seal,Locusta migratoria
Not reactive in
Archeal V-type ATP synthase
Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, includingArabidopsis thalianachloroplastic ATP synthase subunit betaAtCg00480andArabidopsis thalianamitochondrial ATP synthase subunit beta-1At5g08670as well asChlamydomonas reinhardtiiP06541andA8IQU3
Clonality
Polyclonal
Applications
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Dilution
1 : 100 (IF), 1 : 5000 (BN-PAGE), 1 : 2000-1 : 5 000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 10 µl of sterile water
Molecular Weight
53.9 kDa (Arabidopsis thaliana), 51.7 kDa (SynechocystisPCC 6803), 53.7 kDa (Spinacia oleracea)
Precautions
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria,Bacillus cereus.
References & Citations
Soriaet al. (2024).Functional resilience: An active oxidative phosphorylation system prevails amid foreign proteins in holoparasitic plants. Current Plant Biology Volume 37, March 2024, 100322.Cecchinet al (2021). LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Aiharaet al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.Jespersenet al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Rureket al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eomet al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc66fca6/datasheet/as05 085-10.pdf
CAS Number
9007-83-4
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