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Anti-PsbA | D1 protein of PSII, C-terminal, Biotin-conjugated (40 µg)

Product Specifications

Background

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.

Host

Rabbit

Reactivity

Anabaena7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma sp., Coscinodiscus wailesii, Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana,Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Physcomitrium patens, Pinus strobus, rochlorococcus sp. (surface and deep water ecotype), Synechococcus sp.PCC 7942, Spirodela polyrhiza, Symbiodinium sp., Zea mays

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, includingArabidopsis thalianaUniProt:A4QJR4, TAIR:AtCg00020,Oryza sativaP0C434,Populus albaQ14FH6,Physcomitrella patensQ6YXN7,Chlamydomonas reinhardtiiP07753,Synechocystissp.P14660and many others

Clonality

Polyclonal

Applications

Western blot (WB)

Dilution

1 : 15 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.

Format

Liquid

Molecular Weight

38 | 28-30 kDa

Precautions

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the chicken anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.

Storage Conditions

Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70a994c49e6dc66f9c2/datasheet/as05 084b.pdf

CAS Number

9007-83-4

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