Anti-PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Alniaria alnifolia, Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Begonia sp. , Cannabis sativa L., Cladocopium goreaui, Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Chlorella vulgaris, Colobanthus quitensis (Kunth) Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Desmodesmus sp., Dianthus caryophyllus, Ditylum brightwellii, Eucalyptus globulus, Fraxinus rhynchophylla, Glycine max, Gongolaria barbata, Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Manihot esculenta, Marchantia polymorpha (liverwort), Medicago truncatula, Miscanthus x giganteus, Microcystis aeruginosa, Mirkania micrantha, Nicotiana benthamiana, Nicotiana tabcum, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrium patens, Picea glauca, Pinus strobus, Pisum sativum, Prochlorococcus sp. (surface and deep water ecotype), Salicornia bigelovii , Skeletonema costatum (diatom), Solanum lycopersicum, Spartina alterniflora, Spinacia oleracea, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Synechococcus elongatus UTEX 2973, Synechocystis sp. 6803, Syntrichia muralis, Thalassiosira weissflogii, Tetradesmus obliquus, Triticum aestivum, Triticale, Zea may, Quercus ilex

KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.The peptide is conserved in cyanobacterial D1:1 and D1;2.

Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24 kDa and 16 kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.This product can be sold containing ProClin if requested.

Popova et al. (2025) . Differential Photosynthetic Response of Tomato Plants—Ailsa Craig and Carotenoid Mutant tangerine—To Low Light Intensity and Low Temperature Treatment. Crops 2025, 5 (6), 77; doi.org/10.3390/crops5060077. Zhong et al. (2025) . Met246 and Asn250 in the D2 Protein are Essential for the Operation of the Quinone-Fe-Acceptor Complex of Photosystem II. Plant Cell Physiol. 2025 Jul 14:pcaf078. doi: 10.1093/pcp/pcaf078.Lang et al. (2025) . Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early photosystem II assembly factor. Plant Cell. 2025 Jun 4;37 (6) :koaf055. doi: 10.1093/plcell/koaf055. Konishi and Noguchi (2025) . The Mitochondrial Respiratory Chain is Important as an Electron Sink to Avoid Over-Reduction of Chloroplasts in Arabidopsis thaliana Leaves under High-Light Stress. Plant Cell Physiol. 2025 Jun 5:pcaf062. doi: 10.1093/pcp/pcaf062. McKenzie and Puthiyaveetil (2025) . Protein phosphorylation and oxidative protein modification promote plant photosystem II disassembly for repair. Plant Commun . 2025 Mar 10;6 (3) :101202. doi: 10.1016/j.xplc.2024.101202.Huang et al. (2025) . Nitrogen source type modulates heat stress response in coral symbiont (Cladocopium goreaui) . Appl Environ Microbiol. 2025 Feb 19;91 (2) :e0059124. doi: 10.1128/aem.00591-24. Epub 2025 Jan 7. Collombat et al. (2025) . Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis. Commun Biol . 2025 Jan 22;8 (1) :111. doi: 10.1038/s42003-025-07497-y. Pica et al. (2024) . Functional ecological traits in young and adult thalli of canopy-forming brown macroalga Gongolaria barbata (Phaeophyta) from a transitional water system. PeerJ. 2024 Sep 12:12:e17959. doi: 10.7717/peerj.17959. Salazar et al. (2024) . SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii. Nat Commun. 2024 May 20;15 (1) :4279.doi: 10.1038/s41467-024-48595-5.Phukan et al. (2024) . Externally supplied ascorbic acid moderates detrimental effects of UV-C exposure in cyanobacteria. Photochem Photobiol Sci. 2024 Jul 12. doi: 10.1007/s43630-024-00612-8. Zhao et al. (2024) . Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis.Plant J. 2024 May 26. doi: 10.1111/tpj.16844. Völkner et al. (2024) . Evidence for partial functional overlap of KEA and MSL transport proteins in the chloroplast inner envelope of Arabidopsis thaliana. FEBS Lett. 2024 Aug;598 (15) :1877-1887. doi: 10.1002/1873-3468.14887.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Algae (brown and red), Brassica napus, Carica papaya, Conifers, Cyanobacteria, Cannabis sativa, Citrus sinensis, Dicots, Eragrostis tef, Galdieria sulphuraria, Lactuca sativa, Lycopersicum esculentum, Medicago sativa, Nannochloropsis sp., Oryza sativa, Ostreococcus sp., Phalaenopsis aphrodite, Pisum sativum, Porphyridium purpureum, Sesamum indicum, Thalassiosira pseudonana, Zosteria marina, Vitis vinifera cellular [compartment marker] of thylakoid membraneSpecies of your interest not listed? Contact us