Anti-POR | Protochlorophilide oxidoreductase
Selected Variant
AS05 067
Size: 50 µL
Product Specifications
Background
Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
Arabidopsis thaliana, Cyanobacteria, Horderum vulgare, Nicotiana tabacum, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Pinus yunnanensis, Pisum sativum, Triticum aestivum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Native wheat POR protein isolated from a gel piece
Clonality
Polyclonal
Applications
Immunolocalisation (IL), Western blot (WB)
Field of Research
Chlorophyll
Dilution
1: 500 (IL), 1 : 2000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
36-37 kDa (Arabidopsis thaliana)
Precautions
POR is present in high amounts in chloroplasts not exposed to light (etioplasts),
References & Citations
Pavlovič et al. (2024) . Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state. Physiol Plantarum, Volume176, Issue 1.Lee et al (2021) . Chaperone-like protein DAY plays critical roles in photomorphogenesis. Nat Commun. 2021 Jul 7;12 (1) :4194. doi: 10.1038/s41467-021-24446-5. PMID: 34234144; PMCID: PMC8263706.Floris & Kühlbrandt. (2021) . Molecular landscape of etioplast inner membranes in higher plants. Nat Plants. 2021 Apr;7 (4) :514-523. doi: 10.1038/s41477-021-00896-z. Epub 2021 Apr 19. PMID: 33875833.Dogra et al. (2019) . Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019 Jun 27;10 (1) :2834. doi: 10.1038/s41467-019-10760-6. Zhang et al. (2018) . Nitric oxide regulates chlorophyllide biosynthesis and singlet oxygen generation differently between Arabidopsis and barley. Nitric Oxide. 2018 Mar 3;76:6-15. doi: 10.1016/j.niox.2018.03.001.Han et al. (2015) . A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. Plant J. 2015 Jul;83 (2) :277-89. doi: 10.1111/tpj.12889. Epub 2015 Jun 15.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Price
Sign In for Pricing
Documents
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog