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Anti-HSP70 | Salmonid heat shock protein 70, Affinity purified

Product Specifications

Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

100194521

UniProt

B5X4Z3

Host

Rabbit

Reactivity

Oncorhynchus mykiss (Rainbow trout), Danio rerio (Zebrafish)

Not reactive in

Gasterosteus aculeatus

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.

Clonality

Polyclonal

Applications

Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

Field of Research

Fish Proteins

Dilution

5 µg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Molecular Weight

70 kDa

Precautions

This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive

Additionnal Information

Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

References & Citations

Mottola et al. (2020) . Comp Biochem Physiol A Mol Integr Physiol. 2020 Feb;240:110629. doi: 10.1016/j.cbpa.2019.110629. Gallant el al. (2017) . Physiological responses to a short-term, environmentally realistic, acute heat stress in Atlantic salmon, Salmo salar. FACETS.Lewis et al. (2016) . Different Relationship between hsp70 mRNA and hsp70 Levels in the Heat Shock Response of Two Salmonids with Dissimilar Temperature Preference. Front Physiol. 2016 Nov 7;7:511. eCollection 2016.Curie et al. (2008) . β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Coregonus clupeaformis, Oncorhynchus mykiss, Salmo salar (Atlantic salmon), Salvelinus fontinalis (Brook trout)

Documents

Product Datasheet

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