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Anti-Lhca3 | PSI type III chlorophyll a/b-binding protein

Product Specifications

Background

The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

842446

UniProt

Q9SY97

Host

Rabbit

Reactivity

Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Citrus reticulata, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Posidonia oceanica, Spinacia oleracea, Triticum aestivum, Triticale, Zea mays

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

LHC

Dilution

1 : 2000-1 : 5000 (WB)

Purity

Total IgG. Protein G purified in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

29 | 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)

Precautions

Protein is processed into mature form (Jansson1999).

References & Citations

Sarvari et al. (2022) . Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE. Plant Methods. 2022 Mar 3;18 (1) :23. doi: 10.1186/s13007-022-00858-2. PMID: 35241118; PMCID: PMC8895881.Zhu et al. (2020) . A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71 (12) :3613-3625.doi: 10.1093/jxb/eraa118. Chen et al. (2019) . Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21 (1) . pii: E268. doi: 10.3390/ijms21010268.Mao et al. (2018) . Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19 (10) . pii: E3006. doi: 10.3390/ijms19103006.Li et al. (2018) . Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Dicots, Gymnosperms, Glycine max

Frequently Asked Questions

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