Anti-FVIIa | Coagulation factor VIIa (50 µg)

Product Specifications

Background

Factor VIIa (FVIIa) is a key serine protease involved in the initiation of the coagulation cascade. FVIIa requires tissue factor (TF), a membrane bound protein, as an essential cofactor for maximal activity towards its biological substrates Factor X, Factor IX and Factor VII (FVII) .

CAS Number

9007-83-4

HS Code

38221900

Gene ID

835165

UniProt

Q9FI56 , Q9SXJ7

Host

Rabbit

Reactivity

Human

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk A/S Denmark)

Clonality

Polyclonal

Applications

Prothrombin assay with human plasma (PT), thrombin generation assay, inhibition assay with Factor VIIa with TF and a chromogenic substrate, inDirect ELISA (I-ELISA)

Field of Research

Immunology

Dilution

1 : 15 000 (I-ELISA), 14 µg/ml (IL)

Purity

Immunogen affinity purified IgG in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

52 kDa (this is MW of Factor VIIa)

Precautions

This antibody is purified using Protein G, to obtain a total IgG fraction

Additionnal Information

No significant difference in binding to Factor VII and Factor VIIa.Antibody is purified to total IgG fraction, followed by purification on specific FVIIa column.

References & Citations

Lopez-Vilchez et al. (2009) . Traffic of rFVIIa through Endothelial Cells and Redistribution into Subendothelium: Implications for a Prolonged Hemostatic Effect. Journal of Coagulation Disorders, October 1: (1) . (immunolocalization)

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them, to avoid any losses that might occur from material adhering to the cap or sides of the tube.