POU2AF1 Antibody
Goat polyclonal antibody to POU2AF1
Product Specifications
Product Name Alternative
Anti BOB1 antibody, anti OBF1 antibody, anti OBF-1 antibody, anti OCAB antibody, anti POU class 2 associating factor 1 antibody, anti POU domain, class 2, associating factor 1 antibody, anti POU2AF1 antibody
Reactivity
Human
Target
POU2AF1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DSDAYALNHTLSVE
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 1-2 μg/ml, IHC-P: 3-5μg/ml
Molecular Weight
27.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IHC: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5μg/ml.WB: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/ml.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5450
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