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CY5-SE

Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm) :649, Emission (nm) : 670.

Product Specifications

CAS Number

[146368-14-1]

Product Name Alternative

Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/CY5-SE.html

Purity

98.10

Solubility

DMSO : 25 mg/mL (ultrasonic) |H2O : ≥ 5.88 mg/mL

Smiles

CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5

Molecular Formula

C37H43N3O10S2

Molecular Weight

753.88

Precautions

H302, H315, H319, H335

References & Citations

[1]Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88 (10) :5398-5405.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C (Powder, sealed storage, away from moisture and light)

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Citation 01

ACS Appl Mater Interfaces. 2019 Jan 16;11 (2) :1766-1781.|ACS Nano. 2024 Aug 27;18 (34) :23154-23167.|ACS Nano. 2025 May 27;19 (20) :19274-19286.|ACS Omega. 2025 Oct 30;10 (44) :52931-52943.|Adv Funct Mater. 2023 Sep 15.|Adv Healthc Mater. 2025 May 5:e2500511.|Adv Mater. 2025 Jun 13:e2502598.|Adv Sci (Weinh) . 2025 Apr 3:e2415689.|Biomacromolecules. 2019 Jan 14;20 (1) :469-477.|Biomacromolecules. 2025 May 12;26 (5) :3044-3058.|Bioorg Med Chem. 2025 Nov 1:129:118319.|Carbohyd Polym. 2020 May 1;235:115983.|Foods. 2025 Oct 2;14 (19) :3410.|Genomics. 2023 Sep;115 (5) :110703.|Int J Mol Sci. 2025 Feb 13;26 (4) :1594.|J Nanobiotechnology. 2018 Mar 16;16 (1) :23.|J Nanobiotechnology. 2024 Sep 19;22 (1) :578.|J Nanobiotechnology. 2025 May 24;23 (1) :374.|J Pharm Anal. 2025 Apr 14.|J Virol. 2024 May 14;98 (5) :e0035024.|Mol Pharm. 2024 Jul 1;21 (7) :3425-3433.|Molecules. 2024 Sep 10;29 (18) :4293.|Nano Res. 2025 Sep 29.|Nano Res. 29 June 2021.|Nano Today. 2025 Oct.|Research Square Preprint. 2021 Jan.|Adv Sci (Weinh) . 2023 Aug;10 (24) :e2301592.|Int J Biol Macromol. 2024 Jul 17:133957.

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