Recombinant Human Hsp27

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

Hsp27

Gene Name

HSPB1

UniProt

P04792

Cellular Locus

Cytoplasm , Nucleus , Cytoplasm, cytoskeleton, spindle , Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Reactivity

Human

Target Antigen

Heat shock protein beta-1

Target

Hsp27

Type

Protein

Applications

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Form

Liquid

Buffer

20mM HEPES, 1mM DTT, and 100mM KCl.

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.

Storage Conditions

Although stable at 4°C for 1 week, product is best stored at or below -20°C. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.

Specificity

Hsp27

Formulation

Sterile-filtered colorless solution in 20mM HEPES, pH 7.5, 1mM DTT, and 100mM KCl.

Buffer pH

pH 7.5

Target Background

Hsp27, a mammalian heat shock protein, is expressed constitutively in many tissues, and its expression level increases in response to various stress conditions including elevated temperatures, toxic metals, drugs, and oxidants. Hsp27 is phosphorylated in vivo at three phosphorylation sites (Ser15, Ser78, and Ser82) by protein kinases including MAPKAP kinase 2 and stress-activated protein kinase SAPK2.