Anti-Human CD3 Monoclonal Antibody, Flow Validated

Boster Bio Anti-Human CD3 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC02675-1) . Tested in Flow Cytometry application (s) . This antibody reacts with Human.

Product Specifications

Synonyms

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E

Gene Name

CD3E

UniProt

P07766

Host

Mouse

Reactivity

Human

Immunogen

Thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Clonality

Monoclonal

Clone

Clone: Clone: UCHT1

Applications

Flow Cytometry

Field of Research

Adaptive Immunity, Hematopoietic Progenitors, Immunology, Stem Cells, T Cells

Purification

Affinity column chromatography

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

User needs to optimize the dilution ratio for this antibody.

Form

Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098) . In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down- regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128) . .