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Human sVCAM-1/CD106 (soluble Vascular Cell Adhesion Molecule 1) CLIA Kit

Product Specifications

Abbreviation

SVCAM-1/CD106

Cross Reactivity

This kit recognizes Human sVCAM-1/CD106 in samples. No significant cross-reactivity or interference between Human Plg and analogues was observed

Type

Sandwich-ELISA

Applications

CLIA

Assay Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sVCAM-1/CD106. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sVCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sVCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human sVCAM-1/CD106. You can calculate the concentration of Human sVCAM-1/CD106 in the samples by comparing the RLU value of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

0.63-40 ng/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

0.38 ng/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C/-20°C,6 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human sVCAM-1/CD106 concentrations in Serum, plasma and other biological fluids.

Available Sizes

Frequently Asked Questions

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