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TNF-a Tumor Necrosis Factor-Alpha Canine Recombinant Protein

TNF-a Canine Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 17.3 kDa. The TNF-a is purified by proprietary chromatographic techniques.

Product Specifications

Synonyms

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF; TNFA; TNFSF2

Gene Name

TNF

UniProt

P51742

Reactivity

Canine

Source

Escherichia coli

Sequence

[object Object]

Purification

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Bioactivity

5

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Shipping Conditions

Available

Storage Conditions

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Other Gene Names

Tumor necrosis factor

Subcellular Location

Cell membrane

Available Sizes

Frequently Asked Questions

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