Complement Component C5a Human Recombinant Protein

C5a Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8.3kDa. The Human C5a is purified by proprietary chromatographic techniques.

Product Specifications

Synonyms

Complement Component 5; C3 and PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 4; C5a Anaphylatoxin; Prepro-C5; CPAMD4; Anaphylatoxin C5a Analog; ECLZB; C5A; C5D; C5b; C5

Gene Name

UniProt

P01031

Reactivity

Human

Source

Escherichia coli

Sequence

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Purification

Greater than 98.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Bioactivity

The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/ml.

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Reconstitution

Function

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.

Shipping Conditions

Available

Storage Conditions

Lyophilized Human C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.